What is Touchdown (TD)-PCR?
In the touchdown PCR, “By sequentially decreasing the annealing temperature during each PCR cycle, the chance of the non-specific binding can be reduced.” Every PCR technique is evolved to eliminate […]
Genetics
Template DNA in PCR: Function, Properties and Concentration
“Template DNA in PCR is a single-stranded DNA that provides a starting point for amplification. Explore the role, properties and concentration of template DNA and learn how to optimize it.”
Template DNA in PCR: Function, Properties and Concentration Read More »
What is TB-PCR?
“The TB PCR method is the gold standard method in the detection of Tuberculosis infection.” In this method, the insertion sequence of the Tuberculosis is amplified using the polymerase chain
What is in situ PCR?
“The in situ PCR or slide PCR is a method used to amplify the DNA present in the paraffin-embedded tissue or formalin-fixed tissue on the glass slide.” In the
What is Nested PCR?- Concept, Primers, Protocol, Advantages and Limitations
“Nested PCR uses two sets of primers and increases the specificity of the PCR reaction manyfold. And thus reduces the chances of false-positive results.” If someone asks you, what is
What is Nested PCR?- Concept, Primers, Protocol, Advantages and Limitations Read More »
What is a Hot-Start PCR?
“Hot start PCR is a modification of conventional PCR in which limiting one of the reagents until the heating step reduces the chances of non-specific amplification.” Non-specific binding or non-specific