“Nucleic acid extraction is a technique to separate or isolate nucleic acid from a cell. A pure NA is an utmost requirement for downstream molecular analysis such as PCR amplification, […]
Beginners to Advanced Guide for Nucleic Acid Extraction Read More »
“PCR genotyping is a technique to identify pair of alleles present in a sample thereby the condition- homozygous dominant, homozygous recessive or heterozygous.” Thalassemia and sickle cell anemia-like conditions are
6 Optimizations to Improve the PCR Genotyping Read More »
“Nuclease-free water is an ultrapure DNase and RNase-free water used in nucleic acid extraction and PCR reaction preparation.” Nucleic acid extraction/ separation is a process to collect or isolate DNA
4 Important Functions of Nuclease-Free Water Read More »
“Tissue homogenization is a process of preparing a homogenous emulsion or suspension of cells through chemical, physical, mechanical or other ways.” RNA extraction is a ‘bone-crushing’ process and a headache
Tissue Homogenization Techniques for RNA Extraction Read More »
“Only 5% of total cellular RNA content is mRNA and is isolated by removing all other non-coding RNAs from the sample. The unique 3’-OH end of mRNA is utilized to
How to Isolate mRNA from Total Cellular RNA? Read More »
A monophasic solution of guanidinium isothiocyanate and phenol is known as Trizol or Tri-reagent provides stability and integrity to RNA during isolation. Commercially, from company to company the name varies
Effectual Trizol RNA Extraction Protocol using Tri Reagent Read More »
