Role of Guanidine Thiocyanate in RNA extraction
“Guanidine thiocyanate is a standard reagent of RNA lysis buffer and is effective against inactivating proteins. It is also available commercially and we can prepare a working solution in our …
“Guanidine thiocyanate is a standard reagent of RNA lysis buffer and is effective against inactivating proteins. It is also available commercially and we can prepare a working solution in our …
“Nucleic acid extraction is a technique to separate or isolate nucleic acid from a cell. A pure NA is an utmost requirement for downstream molecular analysis such as PCR amplification, …
Beginners to Advanced Guide for Nucleic Acid Extraction Read More »
“RNA degradation is a common molecular lab problem caused by the fragile nature of RNA and the presence of the RNase enzyme predominantly in nature.” Ribonucleic acid-RNA is a one …
“RIN is an RNA Integrity Number showing the value of how intact the RNA is. RIN score >8.0 is ideally recommended for high throughput downstream processing.” Unlike DNA, RNA is …
What is RNA Integrity Number (RIN)? and Why It Is Important? Read More »
“Nuclease-free water is an ultrapure DNase and RNase-free water used in nucleic acid extraction and PCR reaction preparation.” Nucleic acid extraction/ separation is a process to collect or isolate DNA …
“Tissue homogenization is a process of preparing a homogenous emulsion or suspension of cells through chemical, physical, mechanical or other ways.” RNA extraction is a ‘bone-crushing’ process and a headache …
Tissue Homogenization Techniques for RNA Extraction Read More »