RNA: Structure, Types and Function
RNA, Ribonucleic acid is a type of nucleic acid involved in protein synthesis. In the present article, we are briefly discussing each type of RNA with its function and general […]
Genetic Education
Site-Directed Mutagenesis: Methods and Applications
“The site-directed mutagenesis is an artificial (in vitro) technique for introducing mutation or alteration into the target (known) DNA sequence.” In simple words, we can say that by using artificial
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Reverse Transcription PCR: Principle, Procedure, Protocol, Advantages, Limitations, Applications
“RT-PCR can effectively quantify copies of a gene present in the sample. Thus, it’s widely used in gene expression studies, cancer research and viral load estimation.” PCR (Polymerase Chain Reaction)
Real-time PCR: Principle, Procedure, Advantages, Limitations and Applications
“Real-time PCR is a technique used for the quantification of nucleic acid present in the sample using either fluorescence dye or probe-based technique.” PCR is capable enough to amplify the
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Inverse PCR: Principle, Procedure, Protocol and Applications
“Inverse PCR is used for site-directed mutagenesis and insert studies. Learn about the technique, concept, protocol and applications of inverse PCR.” PCR (Polymerase Chain Reaction) is a technique to amplify
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What are PCR Inhibitors and How to Remove Them?
“PCR inhibitors are biological, chemical or other agents that negatively affect the PCR reaction. In this article, learn about various types of PCR inhibitors, the mechanism of inhibition, and how