Role of Guanidine Thiocyanate in RNA extraction
“Guanidine thiocyanate is a standard reagent of RNA lysis buffer and is effective against inactivating proteins. It is also available commercially and we can prepare a working solution in our […]
Beginners to Advanced Guide for Nucleic Acid Extraction
“Nucleic acid extraction is a technique to separate or isolate nucleic acid from a cell. A pure NA is an utmost requirement for downstream molecular analysis such as PCR amplification,
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5 Interpretation Manifests RNA Degradation
“RNA degradation is a common molecular lab problem caused by the fragile nature of RNA and the presence of the RNase enzyme predominantly in nature.” Ribonucleic acid-RNA is a one
What is RNA Integrity Number (RIN)? and Why It Is Important?
“RIN is an RNA Integrity Number showing the value of how intact the RNA is. RIN score >8.0 is ideally recommended for high throughput downstream processing.” Unlike DNA, RNA is
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6 Optimizations to Improve the PCR Genotyping
“PCR genotyping is a technique to identify pair of alleles present in a sample thereby the condition- homozygous dominant, homozygous recessive or heterozygous.” Thalassemia and sickle cell anemia-like conditions are
4 Important Functions of Nuclease-Free Water
“Nuclease-free water is an ultrapure DNase and RNase-free water used in nucleic acid extraction and PCR reaction preparation.” Nucleic acid extraction/ separation is a process to collect or isolate DNA