What is rcDNA?- rcDNA vs cccDNA
“Two common circular forms of DNA are relaxed circular (rc)DNA and covalently closed circular (ccc)DNA. Let us see how both are different and which organisms possess such DNA.” DNA has […]
Why is DNA Extraction From Plants Difficult? (With Possible Solutions)
“Plant species are heterogeneous, contain a differential cell composition and cell wall structure and thus, possibly need a separate DNA extraction protocol for every species.” Plant DNA extraction is a
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Role of Guanidine Thiocyanate in RNA extraction
“Guanidine thiocyanate is a standard reagent of RNA lysis buffer and is effective against inactivating proteins. It is also available commercially and we can prepare a working solution in our
Beginners to Advanced Guide for Nucleic Acid Extraction
“Nucleic acid extraction is a technique to separate or isolate nucleic acid from a cell. A pure NA is an utmost requirement for downstream molecular analysis such as PCR amplification,
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5 Interpretation Manifests RNA Degradation
“RNA degradation is a common molecular lab problem caused by the fragile nature of RNA and the presence of the RNase enzyme predominantly in nature.” Ribonucleic acid-RNA is a one
What is RNA Integrity Number (RIN)? and Why It Is Important?
“RIN is an RNA Integrity Number showing the value of how intact the RNA is. RIN score >8.0 is ideally recommended for high throughput downstream processing.” Unlike DNA, RNA is
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