“Nuclease-free water is an ultrapure DNase and RNase-free water used in nucleic acid extraction and PCR reaction preparation.” Nucleic acid extraction/ separation is a process to collect or isolate DNA […]
4 Important Functions of Nuclease-Free Water Read More »
“Tissue homogenization is a process of preparing a homogenous emulsion or suspension of cells through chemical, physical, mechanical or other ways.” RNA extraction is a ‘bone-crushing’ process and a headache
Tissue Homogenization Techniques for RNA Extraction Read More »
“Only 5% of total cellular RNA content is mRNA and is isolated by removing all other non-coding RNAs from the sample. The unique 3’-OH end of mRNA is utilized to
How to Isolate mRNA from Total Cellular RNA? Read More »
A monophasic solution of guanidinium isothiocyanate and phenol is known as Trizol or Tri-reagent provides stability and integrity to RNA during isolation. Commercially, from company to company the name varies
Effectual Trizol RNA Extraction Protocol using Tri Reagent Read More »
“DEPC (Diethyl pyrocarbonate) is a nuclease inhibitory agent, used in the RNA isolation to inactivate the RNase enzyme.” The single-stranded structure of the RNA makes it more degradative than DNA,
11 Effective Ways to Use DEPC-Treated Water in RNA Isolation Read More »
“RNA extraction is a process to isolate RNA from a cell for gene expression studies and pathogen identification.” RNA extraction is a nucleic acid separation technique to collect various types
21 Things to know for Effective RNA Extraction Read More »
