What is rcDNA?- rcDNA vs cccDNA
“Two common circular forms of DNA are relaxed circular (rc)DNA and covalently closed circular (ccc)DNA. Let us see how both are different and which organisms possess such DNA.” DNA has […]
DNA
Why is DNA Extraction From Plants Difficult? (With Possible Solutions)
“Plant species are heterogeneous, contain a differential cell composition and cell wall structure and thus, possibly need a separate DNA extraction protocol for every species.” Plant DNA extraction is a
Why is DNA Extraction From Plants Difficult? (With Possible Solutions) Read More »
Beginners to Advanced Guide for Nucleic Acid Extraction
“Nucleic acid extraction is a technique to separate or isolate nucleic acid from a cell. A pure NA is an utmost requirement for downstream molecular analysis such as PCR amplification,
Beginners to Advanced Guide for Nucleic Acid Extraction Read More »
4 Important Functions of Nuclease-Free Water
“Nuclease-free water is an ultrapure DNase and RNase-free water used in nucleic acid extraction and PCR reaction preparation.” Nucleic acid extraction/ separation is a process to collect or isolate DNA
5 Important Functions of DNA Supercoiling
DNA supercoiling is a process to make DNA fit inside the cell nucleus and regulates transcriptional activities. But do you know it performs 5 other functions for a cell too?
What is Gel Shift Assay or EMSA?
EMSA stands for Electrophoretic Mobility Shift Assay often known as gel shift assay is a technique to study the nucleic acid and protein interaction. The present technique is used ‘in