“Cell lysis is one of the critical steps in DNA extraction. The process to break a cell wall or nuclear membrane using chemical, physical or enzymatic methods is known as …
How to Prepare Lysis Buffer for Different Types of DNA Extraction Methods? Read More »
Tris and EDTA are two major components which are used throughout the DNA extraction protocol. Tris and EDTA are applicable in lysis buffer preparation, elution buffer preparation and washing buffer …
Importance of Tris-EDTA (TE) Buffer in DNA Extraction Read More »
Different types of DNA purification methods are available for different types of tissue. Alcohol purification, silica gel column based purification, agarose gel-based DNA purification, automated DNA purification and chemical based …
Proteinase K is one of the best DNA extraction methods to date. The method is accurate, reliable and fast. The proteinase K DNA extraction method is also called as an …
Phenol, chloroform and isoamyl alcohol are three basic and important ingredients used in the present method which relies on DNA precipitation using alcohol. It is abbreviated as the PCI DNA …
Phenol Chloroform DNA Extraction: Basics, Preparation of Chemicals and Protocol Read More »
Several different types of DNA extraction methods are Phenol-chloroform isoamyl alcohol, Proteinase K, CTAB method, spin column-based methods and magnetic bead-based technique. However, which method to use depends on the …
