“Cell lysis is one of the critical steps in DNA extraction. The process to break a cell wall or nuclear membrane using chemical, physical or enzymatic methods is known as…
Continue readingTris and EDTA are two major components which are used throughout the DNA extraction protocol. Tris and EDTA are applicable in lysis buffer preparation, elution buffer preparation and washing buffer…
Continue readingDifferent types of DNA purification methods are available for different types of tissue. Alcohol purification, silica gel column based purification, agarose gel-based DNA purification, automated DNA purification and chemical based…
Continue readingProteinase K is one of the best DNA extraction methods to date. The method is accurate, reliable and fast. The proteinase K DNA extraction method is also called as an…
Continue readingPhenol, chloroform and isoamyl alcohol are three basic and important ingredients used in the present method which relies on DNA precipitation using alcohol. It is abbreviated as the PCI DNA…
Continue readingSeveral different types of DNA extraction methods are Phenol-chloroform isoamyl alcohol, Proteinase K, CTAB method, spin column-based methods and magnetic bead-based technique. However, which method to use depends on the…
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