What is a Long-Range PCR?
“Long-range PCR is a method to amplify the DNA fragment of more than 5Kb using a special type of high-fidelity DNA polymerase.” Using the PCR ingredients like dNTPs, PCR buffer, […]
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Genetics
Colony PCR- Principle, Process, Protocol, Advantages and Limitations
“A rapid colony screening method, the colony PCR validates the target DNA or gene construct present in a bacteria that has grown on selective media.“ As a result, researchers determine
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What is an In Silico PCR? How to Perform it?
“An in silico PCR is a computational tool used to determine the results of practical PCR reaction computationally using a set of the selected primers.” Here in the in silico
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Advanced Guide for the DNA Extraction Lab Setup
“Explore this advanced guide to set up the DNA extraction lab for research, diagnosis and other activities. Learn about the requirements and utilities needed.” The basic DNA extraction setup used
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What is Touchdown (TD)-PCR?
In the touchdown PCR, “By sequentially decreasing the annealing temperature during each PCR cycle, the chance of the non-specific binding can be reduced.” Every PCR technique is evolved to eliminate
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Template DNA in PCR: Function, Properties and Concentration
“Template DNA in PCR is a single-stranded DNA that provides a starting point for amplification. Explore the role, properties and concentration of template DNA and learn how to optimize it.”
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