What is Gradient PCR and How to Use it?
“In a gradient PCR, we can create temperature variation for the annealing step, set different annealing temperatures and test different parameters at different temperatures.” PCR is an amazing discovery in […]
Genetics
A Guide to Choose DNA Polymerase for Your Experiment
“Countless commercially available DNA polymerases make the choice difficult. Here is the guide on how to select a DNA Polymerase for your genetic experiment.” The function of a DNA polymerase
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Site-Directed Mutagenesis: Methods and Applications
“The site-directed mutagenesis is an artificial (in vitro) technique for introducing mutation or alteration into the target (known) DNA sequence.” In simple words, we can say that by using artificial
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Reverse Transcription PCR: Principle, Procedure, Protocol, Advantages, Limitations, Applications
“RT-PCR can effectively quantify copies of a gene present in the sample. Thus, it’s widely used in gene expression studies, cancer research and viral load estimation.” PCR (Polymerase Chain Reaction)
Real-time PCR: Principle, Procedure, Advantages, Limitations and Applications
“Real-time PCR is a technique used for the quantification of nucleic acid present in the sample using either fluorescence dye or probe-based technique.” PCR is capable enough to amplify the
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Inverse PCR: Principle, Procedure, Protocol and Applications
“Inverse PCR is used for site-directed mutagenesis and insert studies. Learn about the technique, concept, protocol and applications of inverse PCR.” PCR (Polymerase Chain Reaction) is a technique to amplify
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