“Primer dimers are unintended short amplification products formed in the PCR by unhealthy PCR practices. This article covers common causes for primer dimer formation and troubleshooting.” In the previous article […]
PCR Troubleshooting 103: How to Address Primer-Dimers Read More »
“Allelic dropout in the PCR is a condition in which one or both the alleles in the heterozygous condition fail to amplify and cause a fault in results. In this
PCR Troubleshooting 102: How to Address The Allelic Dropout Read More »
“Non-specific amplification is one of the most common problems in any PCR reaction. In this guide, I will explain the reasons and possible (technical) solutions to overcome non-specific amplification.” Imagine,
PCR Troubleshooting 101: How to Address Non-Specific Amplification? Read More »
“Sanger sequencing is a genetic technique to determine the order of nucleotides while PCR generates copies of the DNA fragment, present in the sample. Let’s see some of the significant
Sanger Sequencing vs PCR: Common and Technical Differences Read More »
“PCR genotyping is a technique to identify pair of alleles present in a sample thereby the condition- homozygous dominant, homozygous recessive or heterozygous.” Thalassemia and sickle cell anemia-like conditions are
6 Optimizations to Improve the PCR Genotyping Read More »
“PCR amplification is an enzyme-dependent process to copy a DNA sequence, DNA fragment, genomic DNA, plasmid DNA or any nucleic acid.“ In other words, we can say, Producing multiple copies
10 Strategies to Achieve Excellent PCR Amplification Read More »
