What is a Palindromic DNA Sequence?
“A sequence when read from 5’ to 3’ and 3’ to 5’ direction on two antiparallel strands shows sequence similarity is said as the palindromic DNA sequence.” Our genome, in […]
What is Gel Shift Assay or EMSA?
EMSA stands for Electrophoretic Mobility Shift Assay often known as gel shift assay is a technique to study the nucleic acid and protein interaction. The present technique is used ‘in
What is DNA footprinting?- Principle, Steps, Process and Applications
“DNA footprinting studies the DNA sequence protein interaction thereby transcriptional activities in vitro within or outside the cell.“ Transcription is a complex biological process whose final product is obviously a
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Cas9 Protein: Structure, Function, Types and Importance
“The Cas9 protein is a nuclease that destroys phage DNA using the double RNA-guided DNA cleavage through DNA binding and nuclease activities.” Key points: Cas9 protein is predominantly present in
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Genomic DNA Library- Preparation and Applications
A genomic library is a collection of clones/copies of smaller-sized genomic DNA fragments, used in various downstream applications. Do you know? The human genome size is ~3.2 billion base pairs.
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How does the CRISPR CAS9 System Distinguish Self vs Nonself DNA?- 7 Proven mechanisms
“CRISPR works magically in every sense! Every time scientists doubt something and do research, they get more than that, something new and amazing. Yet, it’s not so complex.” Do you
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