qPCR acronym as quantitative PCR is a technique to measure the amount of DNA present in a sample. It can study gene expression by utilizing either the TaqMan probe or […]
Easiest qPCR Protocol using TaqMan Probe and SYBR Green Dye Read More »
An exclusive native PCR modification known as Asymmetric PCR has been applied to amplify only a single strand during the PCR. PCR is a thermocycler, amplifies DNA. Meaning, it helps
Asymmetric PCR: Principle, Process, Protocol, Applications, Advantages and Limitations Read More »
“The percent of Guanine and Cytosine, nitrogenous bases present in a DNA, RNA, nucleic acid sequence or primer is known as GC content. The unit to calculate the GC content
What is the importance of GC Content? Read More »
Learn about PCR controls– Positive, Negative and internal controls, how to ensure accuracy & reliability, and why we use them in molecular genetic studies. PCR (Polymerase Chain Reaction) is a
Type of PCR Controls- Negative, Positive and Internal Controls Read More »
The annealing temperature is the temperature at which the PCR primers bind to the complementary template region, usually between 50ºC to 68ºC. Learn about annealing temperature in this article. We have
What is Annealing Temperature in PCR? How to Calculate and Set It? Read More »
Usually, 25 to 35 cycles a typical PCR reaction needs to get a good amount of amplification, containing denaturation, annealing and extension simultaneously at 95ºC, 50 to 68ºC and 72ºC.
Optimizing PCR Cycles to Get Good Results Read More »
