An exclusive native PCR modification known as Asymmetric PCR has been applied to amplify only a single strand during the PCR. PCR is a thermocycler, amplifies DNA. Meaning, it helps
Asymmetric PCR: Principle, Process, Protocol, Applications, Advantages and Limitations Read More »
“The percent of Guanine and Cytosine, nitrogenous bases present in a DNA, RNA, nucleic acid sequence or primer is known as GC content. The unit to calculate the GC content
Learn about PCR controls– Positive, Negative and internal controls, how to ensure accuracy & reliability, and why we use them in molecular genetic studies. PCR (Polymerase Chain Reaction) is a
Type of PCR Controls- Negative, Positive and Internal Controls Read More »
The annealing temperature is the temperature at which the PCR primers bind to the complementary template region, usually between 50ºC to 68ºC. Learn about annealing temperature in this article. We have
What is Annealing Temperature in PCR? How to Calculate and Set It? Read More »
Usually, 25 to 35 cycles a typical PCR reaction needs to get a good amount of amplification, containing denaturation, annealing and extension simultaneously at 95ºC, 50 to 68ºC and 72ºC.
“Mitochondrial and X chromosomal DNA, traits like fetus’ gender & intelligence and related-diseases are inherited from the mother.” The present article has very interesting information on how genetic inheritance differs
What Genes, Traits and Disorders are inherited from the Mother only? Read More »
