Setup a Genetic Testing Lab in 12 Easy Steps
Genetic testing labs can report genetic abnormality by investigating DNA, gene or chromosomes, generate a report and can advise management steps. Before a decade, it was difficult to run a […]
Easiest qPCR Protocol using TaqMan Probe and SYBR Green Dye
qPCR acronym as quantitative PCR is a technique to measure the amount of DNA present in a sample. It can study gene expression by utilizing either the TaqMan probe or
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Asymmetric PCR: Principle, Process, Protocol, Applications, Advantages and Limitations
An exclusive native PCR modification known as Asymmetric PCR has been applied to amplify only a single strand during the PCR. PCR is a thermocycler, amplifies DNA. Meaning, it helps
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What is the importance of GC Content?
“The percent of Guanine and Cytosine, nitrogenous bases present in a DNA, RNA, nucleic acid sequence or primer is known as GC content. The unit to calculate the GC content
Type of PCR Controls- Negative, Positive and Internal Controls
Learn about PCR controls– Positive, Negative and internal controls, how to ensure accuracy & reliability, and why we use them in molecular genetic studies. PCR (Polymerase Chain Reaction) is a
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What is Annealing Temperature in PCR? How to Calculate and Set It?
The annealing temperature is the temperature at which the PCR primers bind to the complementary template region, usually between 50ºC to 68ºC. Learn about annealing temperature in this article. We have
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