What is the importance of GC Content?
“The percent of Guanine and Cytosine, nitrogenous bases present in a DNA, RNA, nucleic acid sequence or primer is known as GC content. The unit to calculate the GC content […]
Type of PCR Controls- Negative, Positive and Internal Controls
Learn about PCR controls– Positive, Negative and internal controls, how to ensure accuracy & reliability, and why we use them in molecular genetic studies. PCR (Polymerase Chain Reaction) is a
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What is Annealing Temperature in PCR? How to Calculate and Set It?
The annealing temperature is the temperature at which the PCR primers bind to the complementary template region, usually between 50ºC to 68ºC. Learn about annealing temperature in this article. We have
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Optimizing PCR Cycles to Get Good Results
Usually, 25 to 35 cycles a typical PCR reaction needs to get a good amount of amplification, containing denaturation, annealing and extension simultaneously at 95ºC, 50 to 68ºC and 72ºC.
What Genes, Traits and Disorders are inherited from the Mother only?
“Mitochondrial and X chromosomal DNA, traits like fetus’ gender & intelligence and related-diseases are inherited from the mother.” The present article has very interesting information on how genetic inheritance differs
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What is Post-Translational Modifications (PTMs)?- 10 Most Commonly Studied PTMs
“Post-translational modifications, abbreviated s PTMs are the biological modifications that occur after protein synthesis which alters protein structure and function. Common PTMs are phosphorylation, Methylation, acetylation, lipidation, hydroxylation and ubiquitination.
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