What is CIRSPRa or CRISPR activation?
CRISPRi or CRISPR activation is a process to upregulate gene expression or activate genes by effector activators, dcas9 and sgRNA. In 2021, CRISPR have deep penetration in diverse scientific fields. …
CRISPR-CAS9
What is CRISPRi or CRISPR interference?
CRISPRi, short for CRISPR interference, is designed to repress gene expression using the customized sgRNA and dCAS. CRISPR- CAS9 is simply amazing! Why? Because it can edit a gene at …
dCAS9 (Dead CAS system): Concept, Functions and Applications
“dCAS9 is a deactivated or dead Cas protein that lacks nucleophilic activity or simply is a deactivated nuclease CAS9.” Read more: CRISPR-Cpf1(Cas12a) Gene-Editing System- Explained. You may wonder, Without nucleophilic …
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Applications of CRISPR-CAS9 in Medical Science, Diagnostics, Research, Plant Biology and Agriculture
“CRISPR CAS9 is a revolutionary recombinant/ gene-editing technique used in medical research, disease diagnosis, plant research and agriculture.” The era of recombinant DNA technology or more precisely we can say …
The Curious Case of CRISPR-Babies
“Chinese scientists had used CRISPR-like tools to edit the live embryo. By editing germ cells they had produced genetically altered babies and who are known as CRISPR-babies.” The story of …
What is sgRNA in CRISPR-CAS9?- Definition, Designing and Advantages
“The sgRNA or gRNA is known as single-stranded guided RNA or guided RNA used in the gene-editing technique which makes a complex with the CAS9 protein to cleave the DNA.” …
What is sgRNA in CRISPR-CAS9?- Definition, Designing and Advantages Read More »
