What is CIRSPRa or CRISPR activation?
CRISPRi or CRISPR activation is a process to upregulate gene expression or activate genes by effector activators, dcas9 and sgRNA. In 2021, CRISPR have deep penetration in diverse scientific fields. […]
CRISPR-CAS9
CRISPR-Cas9 is a revolutionary gene editing technology, inspired by the natural bacterial defense system. Due to its high precision, efficiency and ability to edit the DNA, it has been widely used in medicine, agriculture and other scientific fields. Learn the fundamentals, components, process and steps, and applications of CRISPR technology in this category.
Explore all the articles on CRISPR-Cas9 and improve your gene editing knowledge.
What is CRISPRi or CRISPR interference?
CRISPRi, short for CRISPR interference, is designed to repress gene expression using the customized sgRNA and dCAS. CRISPR- CAS9 is simply amazing! Why? Because it can edit a gene at
dCAS9 (Dead CAS system): Concept, Functions and Applications
“dCAS9 is a deactivated or dead Cas protein that lacks nucleophilic activity or simply is a deactivated nuclease CAS9.” Read more: CRISPR-Cpf1(Cas12a) Gene-Editing System- Explained. You may wonder, Without nucleophilic
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Applications of CRISPR-CAS9 in Medical Science, Diagnostics, Research, Plant Biology and Agriculture
“CRISPR CAS9 is a revolutionary recombinant/ gene-editing technique used in medical research, disease diagnosis, plant research and agriculture.” The era of recombinant DNA technology or more precisely we can say
The Curious Case of CRISPR-Babies
“Chinese scientists had used CRISPR-like tools to edit the live embryo. By editing germ cells they had produced genetically altered babies and who are known as CRISPR-babies.” The story of
What is sgRNA in CRISPR-CAS9?- Definition, Designing and Advantages
“The sgRNA or gRNA is known as single-stranded guided RNA or guided RNA used in the gene-editing technique which makes a complex with the CAS9 protein to cleave the DNA.”
What is sgRNA in CRISPR-CAS9?- Definition, Designing and Advantages Read More »