“A hairpin-shaped, single-stranded, sequence-specific real-time PCR probe is known as Molecular Beacon having a higher sensitivity and specificity.” The present article is more technical, including in-depth technical things related to […]
Structure and Function of Molecular Beacon Probes Read More »
“A typical PCR reaction contains a required concentration of dNTPs, Taq DNA polymerase, PCR primers, PCR buffer, template DNA and water. Each PCR reaction varies in terms of the concentration
How to Prepare an Excellent PCR Reaction? A step-by-step Guide Read More »
“DNA precipitation is a process of nucleic acid (DNA/ RNA) precipitation using alcohol and salt. Ethanol and isopropanol are the two most common types of alcohol used in the DNA
A Quick Guide on DNA Precipitation and Protocol Read More »
“Primer dimers are short amplified products formed by the binding of primers during PCR amplification. Let’s learn what primer dimers are in PCR, qPCR, gel electrophoresis and DNA sequencing.” PCR
What are Primer Dimers? – A Beginner’s Guide Read More »
“In a gradient PCR, we can create temperature variation for the annealing step, set different annealing temperatures and test different parameters at different temperatures.” PCR is an amazing discovery in
What is Gradient PCR and How to Use it? Read More »
“Countless commercially available DNA polymerases make the choice difficult. Here is the guide on how to select a DNA Polymerase for your genetic experiment.” The function of a DNA polymerase
A Guide to Choose DNA Polymerase for Your Experiment Read More »
