“Only 5% of total cellular RNA content is mRNA and is isolated by removing all other non-coding RNAs from the sample. The unique 3’-OH end of mRNA is utilized to […]
How to Isolate mRNA from Total Cellular RNA? Read More »
A monophasic solution of guanidinium isothiocyanate and phenol is known as Trizol or Tri-reagent provides stability and integrity to RNA during isolation. Commercially, from company to company the name varies
Effectual Trizol RNA Extraction Protocol using Tri Reagent Read More »
“DEPC (Diethyl pyrocarbonate) is a nuclease inhibitory agent, used in the RNA isolation to inactivate the RNase enzyme.” The single-stranded structure of the RNA makes it more degradative than DNA,
11 Effective Ways to Use DEPC-Treated Water in RNA Isolation Read More »
“RNA extraction is a process to isolate various or a specific type of RNA molecules for gene expression studies.” Download this article as an ebook: RNA Extraction pdf. Snippet: Like
RNA Extraction: Principle, Procedure, Protocol and Importance Read More »
“Microsatellite instability is defined as indel (insertion or deletion) mutations within the repetitive regions (microsatellite) in cancer cells by the inactivation of DNA mismatch repair proteins.“ Do you know? Introns
Role of Microsatellite Instability (MSI) in cancer (MSI-H, MSI-I and MSS) Read More »
“Loss of Heterozygosity (LOH) is defined as the absence of one normal parental allele or gene (or part of the chromosome) in an organism which usually results in cancer.“ Like
What is Loss of Heterozygosity? How It is Associated with Cancer? Read More »
