What is rcDNA?- rcDNA vs cccDNA
“Two common circular forms of DNA are relaxed circular (rc)DNA and covalently closed circular (ccc)DNA. Let us see how both are different and which organisms possess such DNA.” DNA has […]
Genetic Education
Why is DNA Extraction From Plants Difficult? (With Possible Solutions)
“Plant species are heterogeneous, contain a differential cell composition and cell wall structure and thus, possibly need a separate DNA extraction protocol for every species.” Plant DNA extraction is a
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Beginners to Advanced Guide for Nucleic Acid Extraction
“Nucleic acid extraction is a technique to separate or isolate nucleic acid from a cell. A pure NA is an utmost requirement for downstream molecular analysis such as PCR amplification,
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6 Optimizations to Improve the PCR Genotyping
“PCR genotyping is a technique to identify pair of alleles present in a sample thereby the condition- homozygous dominant, homozygous recessive or heterozygous.” Thalassemia and sickle cell anemia-like conditions are
4 Important Functions of Nuclease-Free Water
“Nuclease-free water is an ultrapure DNase and RNase-free water used in nucleic acid extraction and PCR reaction preparation.” Nucleic acid extraction/ separation is a process to collect or isolate DNA
Tissue Homogenization Techniques for RNA Extraction
“Tissue homogenization is a process of preparing a homogenous emulsion or suspension of cells through chemical, physical, mechanical or other ways.” RNA extraction is a ‘bone-crushing’ process and a headache
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