All articles, Educational articles, Explore, MutationDifferent types of Genetic mutations

Different types of Genetic mutations

Different types of Genetic mutations

Chromosomal aberrations, point mutation, deletion of nucleotides and addition of nucleotides, loss of function and gain of function are some of the examples of different type of Genetic mutationsThe term mutation was coined by Hugo De Vries in 1890.

Mutation is an important phenomenon in nature for the creation of variation.

“Under the influence of environment and other adverse conditions, any alteration in nucleotide sequences of DNA is called as mutations”.

Different types of Genetic mutations are occurred due to our adverse lifestyle. In routine life, we are facing so many unfavourable conditions such as adverse medication, contrast food, UV light radiations and other adverse environmental conditions.

Many different nucleotides are deleted or inserted during adverse conditions but these are non-pathogenic. However, some conditions are extreme, as in SRY, if some sequences or entire SRY gene is deleted, individual remain infertile, during entire life.

Different types of Genetic mutations are explained here. The outline of the topic is as followed,

Different types of Genetic mutations: 

  • Spontaneous vs Induced mutation
  • Chromosomal mutation vs mitochondrial mutation
  • Somatic vs germline mutation
  • Morphological mutation
  • Lethal mutation
  • Conditional mutation
  • Biochemical mutation
  • Loss of function mutation
  • Gain of function mutation
  • Temperature sensitive mutation
  • Gene mutations

When a single nucleotide is deleted or inserted in a genome, it creates SNPs (single nucleotide polymorphism). Millions of different SNPs are arising every day but DNA has its own repair mechanism, which repairs almost any variation of the genome.

Any alteration in a nucleotide sequence of DNA is called as a mutation. At a molecular level, single nucleotide to several nucleotides may alter. Nevertheless, at the chromosomal level, entire segment or several gene segments can alter. The alteration may be addition, deletion, substitution or translocation. Broadly we can categorize different types of Genetic mutations as conditional mutations, Chromosomal mutations or Gene mutations. 

Different types of Genetic Mutations:

Spontaneous vs Induced mutation

A mutation which occurred without any known cause is called as a spontaneous mutation. It arises due to metabolic errors, replication errors or during development errors. Spontaneous mutations are rare and occurred without any reason. Spontaneous mutations are generally occurred by birth.

Larger genes are more prone to spontaneous mutation because as the gene is larger the chance of error in replication is higher.  The rate of spontaneous mutation is 10-5 per gene per generation during replication. For more detail on replication refer our articles,

DNA Replication class 1: General process of DNA replication

Prokaryotic DNA replication: Replication class 2

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The image represents induced vs spontaneous mutation.

Induced mutation is resulting from exposure of an organism to mutagenic agents. The general mutagenic agents are radiation, UV light and chemicals.

UV light is responsible for xeroderma pigmentation and skin cancer by penetrating into the skin. Because UV light cannot penetrate inside the body due to it’s lower energy. Although it cannot damages other tissue of our body, it can damage skin cells. Chemicals such as alkylating agents and base analogues are common chemical mutagens. However, every chemical is somehow mutagenic to our DNA.

Additionally,  environmental changes and lifestyle of an organism may result in some of the mutations. Spontaneous genetic mutations occur infrequently in every generation but, are stably inherited into the population. However, the rate of inheritance depends on the types of inheritance pattern. 

For more detail on inheritance, read our article: different types of inheritance pattern

“Under the influence of environment and other adverse conditions, any alteration in nucleotide sequences of DNA is termed as mutations”. 

Although spontaneous Genetic mutation does not show any sign or symptoms in the parental/ affected organism or (remain recessive), it can stably express in further consecutive generations.

In contrast, induced Genetic mutation immediately shows their effect in an organism who is being affected with mutagens.

Somatic vs germ-line mutation

Mutations which are occurred in the somatic cells of an organism is called as a somatic mutation. Somatic mutations are non inherited because only germ cells (sperm or egg ) can only undergo fertilization. Though it is non inherited, it can still cause some life-threatening phenotypes.

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Mutation in somatic cells

Somatic mutation is restricted to some tissue or some part of the body. But if it occurs in progenitor cells it can be spread to other daughter cells. Progenitor cells are the group of cells which have the capacity to divide further.

The commonest type of somatic mutation results in cancer. In contrast, germline mutation inherits from one generation to other generation. Two types of germ cells sperm and egg, have haploid numbers of a genome and undergo fertilization. Hence if it occurs in germ cells it is inherited into the next generation.

In somatic mutation, if the mutation occurs in cells after metaphase, it can not be spread to the other cells. The somatic mutation occurs during the mitosis whereas germline mutation occurred during meiosis.

Germline mutations may or may not affect the parental organism but it will surely affect the offsprings. However, germline mutations are generally non-curable. All the inherited disorders are germline mutations.

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The image represents somatic vs germline mutation

Actually, germline mutation provides additional diversity in population but it may be harmful to the organism, sometimes. In contrast, somatic mutation always affects an organism since the origin of the mutation and it can not produce diversity.

Somatic mutations are restricted to an individual, if it is lethal, an individual may die but it cannot inherent to other organisms in the population. Whereas germline mutation is silent, it remains in a recessive condition in some organism and steadily spreads throughout the population.

Chromosomal mutation vs mitochondrial mutation

Mitochondria are the powerhouse organelle of our cell. Mitochondrial DNA (mtDNA) is present in mitochondrial (not in the nucleus), and it has its own replication and transcription machinery. 

Genomic mutations are inherited in Mendelian fashion. It is inherited in a syndromic manner. Whereas mitochondrial mutations are non-syndromic and do not follow Mendelian inheritance.

One of the interesting thing about mtDNA is, it is inherited from mother. Though all the cells of our body have mitochondria, it is inherited from our mother to us. So it is called as maternally inherited DNA. If the mutation is inherited to male, it cannot further be transmitted.

Read further,

  1. Extrachromosomal inheritance class 2: organelle DNA
  2. Extrachromosomal inheritance class 1: Introduction to maternal inheritance

Chromosomal mutations may inherited in Mendelian fashion. It inherited to any of the offspring depending upon the type of inheritance. However, Cry du chat, down syndrome and patau syndrome are some of the common types of chromosomal disorders which do not follows Mendelian inheritance. 

Mitochondrial DNA is inherited maternally, it is rarest among rare. Kearns- syre syndrome, Leigh syndrome and non-syndromic hearing loss are some of the mitochondrial disorders which arise due to the mutation in mtDNA.

Deletion, duplication, translocation and inversion is a common type of mutations observed in mtDNA same as genomic DNA. 

Chromosomal mutation

At the molecular level, single nucleotides to several hundred nucleotides are altered but at the chromosomal level large segment of the chromosome with one gene to many, are deleted or added. Depending upon the type of action, chromosomal mutations are categorized into addition, deletion, duplication, translocation, inversion, ring chromosome.

Addition: when some of the extra segment is added to a chromosome, is called as an addition.

Deletion: when some segment of an entire chromosome is deleted and losses several genes, it is called as a deletion.

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The image is a representation of chromosomal mutation

Duplication: one segment of a chromosome is duplicated in a manner which alters the structural hierarchy of the entire chromosome and creates chromosomal abnormality is called as a chromosomal duplication.

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chromosomal duplication

Translocation

Balanced translocation: when two segments (nearly same) are exchanged, resulting in balanced translocation. Here the two segments are approximately the same in size hence it is not possible to detect such type of translocation by cytogenetic analysis.

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The image represents a general type of chromosomal translocation.

Generally, balanced translocation results in recurrent abortion in females and it results in abnormalities in the fetus. Balance translocation can be screened by FISH.

Reciprocal translocation: translocation occurred between two non-homologous chromosomes, is called as reciprocal translocation. This type of translocation is most common in nature.

Robertsonian translocation: Robertsonian translocation occurs between two acrocentric chromosomes. Acrocentric chromosomes are small short chromosome with one long q-arm and a short or very smaller p-arm.

In humans 13, 14, 15, 21, 22 and Y chromosomes are acrocentric. When translocation between two acrocentric chromosomes occurs, one (nearly) metacentric and one “only centromeric” chromosomes are formed.

Here long arms of both chromosome are fused together. Therefore, one chromosome become larger metacentric and another chromosome remain without any arms or only with centromere.

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Different type of chromosomal translocation.

Though the “only centromeric” chromosome does not have any chromatid, still it contains many genes of both chromosomes. Balanced Robertsonian translocation does not cause any physical abnormality but recurrent abortion in females is the common abnormality.

Imbalanced Robertsonian translocation results in mental retardation, trisomy and other structural abnormalities.

Inversion: a segment of a chromosome is inverted and rearranged back to the parental chromosome, is called as an inversion. Here if the inversion occurs in only one arm of the chromosome, this type of inversion is called as a paracentric inversion. The centromere is not involved in paracentric inversion.

If the inversion occurs between two arms of a chromosome, this type of inversion is called as a pericentric inversion. The centromere is involved in pericentric inversion.

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The image represents a general type of chromosomal inversion.

Ring chromosome: when both ends of a chromosome are fused together, it creates a ring-like structure and so-called as ring chromosome. A ring chromosome is generally observed in the long metacentric chromosomes.

Telomeres of each chromosome have specific sequences which prevent them to fuse together. When some of this sequences (telomeric sequences)are losses result in fusion of chromosomal ends.

Ring chromosome results in wide varieties of phenotypic abnormalities like microcephaly, mental retardation and epilepsy.

Morphological mutation

The Genetic mutation which affects the outer characteristic or physical characteristic of an organism is called as a morphological mutation. This type of mutation alters the physical properties like shape, size and colour of an organism.

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Morphological mutation

Lethal mutation

A Genetic mutation which causes the death of an organism or affects the survival of an organism is called as a lethal mutation. If a mutation causes death in a certain environment than the mutation is called as a conditional lethal mutation.

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Lethal genetic mutation

Conditional mutation

In this type of Genetic mutations, the mutant allele causes mutant phenotype in a certain specific environment while remaining wild type in some other environment.

Let’s take the example of bacteria,

The conditions which favour the growth of mutant colonies are called restrictive conditions.  In contrast, conditions which cause the growth of wild-type phenotype (having wild-type allele) are called as permissive conditions.

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Conditional genetic mutations

Conditional mutation is very important in genetic studies. We can study the characteristic of an allele, and how it behaves in different adverse conditions. We can estimate the time duration of gene action as well. 

Biochemical mutation

Each cell required energy and nutrients for differentiation and survival. Different biochemical pathway inside the cell provides that essential requirement. For mutation study, bacteria are the most suitable model organism because of their unique properties.

Bacteria or microbial culture which can grow on minimal media (only simple inorganic salts) are called as prototrophic. In contrast, the mutant bacterial culture which required all the essential nutrients like amino acids and other organic salts, are called as auxotrophic.

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Biochemical Genetic mutation

Wild-type cultures are prototrophic while biochemical mutants are auxotrophic in nature Because biochemical mutant lacks in some metabolites which they required to grow. Hence they need all type of nutrients.

Auxotrophic mutants are unable to synthesize essential nutrients like amino acid, vitamins and nitrogenous bases whereas wild-type strains of bacteria can synthesize all the essential metabolites.

When nutrients and metabolites rich media are given to auxotrophic mutants, the condition has become permissive for them and mutant colonies can grow faster.   

Loss of function mutation

In simple terms, a mutation that causes functional retardation of the gene are called as loss of function mutation. Loss of function mutation depends on the condition of inheritance of that mutation. Generally, it remains recessive.

If the wild-type normal allele is dominant and expressed over mutant allele then even in heterozygous condition, the loss of function mutation remains recessive. It is also called a null mutation.

Mutant allele is expressed in a condition where both the recessive alleles (homozygous recessive) are overexpressed as compared to the normal wild-type allele.

Gain of function mutation

Loss of function Genetic mutations are most common in nature, but some mutation gives new function to the gene or gives original function back to the gene. However, this types of mutation are rare. Remember, sickle allele.

In sickle cell anaemia-heterozygous condition, individual remain unaffected but mutation gives one additional benefit. it protects against the malaria parasite. However, in the homozygous mutant condition, it causes anaemia. 

Temperature sensitive mutant

Mutant that is growing at one temperature and remain suppressed at another temperature, are called as a temperature-sensitive mutation. Most enzyme-coding genes are temperature sensitive, enzymes are activated at once specific temperature.

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A temperature sensitive condition for mutant and wild-type colonies.

 

Gene mutations

Forward mutation

Genetic Mutation from wild-type to mutant or evolution of new mutation from wild-type is called as a forward mutation. Forward mutation leads to an evolution of new traits in the population. It is a natural phenomenon in nature.

Backward mutation

Mutation is a unidirectional process, but sometimes some mutation gives original (wild-type) traits back to population, such mutation is called as the backward or back mutation. Back mutation is very rare and unusual process in nature.

It gives original phenotype back into the population by true back mutation or by the occurrence of a secondary mutation.

In true back mutation, a mutation occurred at the same location as it occurred during forward mutation. In simple words, it gives the wild-type codon back to population.

In secondary mutation, an occurrence of a new mutation in any place in the sequence of the gene which gives the original function back or sometimes the new mutation suppressed the effect of the previous forward mutation.

Copying error

Copying error occurs during cell division while replication. If it remains un-repaired, such a mutation will change the genetic code.

Point mutation: mutation in which a single base is altered, termed as a point mutation (addition or deletion of a single nucleotide). 

We had already covered an article on the nucleotide, read the article here: The Function of dNTPs in PCR reaction

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The image represents a point mutation in the DNA sequence. image credit: genome research limited

Silent mutation: it is non-expressive. In silent mutation, the new codon is created from the mutation but it codes for the same amino acid as wild-type.

Mis-sense mutation: A codon originated from a nucleotide change that will code for different amino acids. It can lead to alteration or loss of function in protein.

Non-sense mutation: A stop codon is added to the premature protein. It stops protein synthesis because a stop codon ends synthesis of protein and results in a premature protein.

Frameshift mutation: Alteration in a base pair which results in an abnormal reading frame, and leads to abnormal protein. A specific reading frame has a start codon and a stop codon, in between both codons, a definite coding sequence is present. In frameshift mutation, alteration in DNA  leads to shifting of this reading frame hence the position of start or stop codon altered which results in an abnormal protein.

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The image represents frameshift in the DNA sequence. image credit: genome research limited

Some of the mutations are extra large; in fragile X syndrome, an alteration in triple repeat number results in an abnormal protein. In case of DMD, an entire exon is deleted from DMD gene. Some mutations can alter the expression of other genes. For example, a mutation in the SOX9 somatic gene results in the altered expression of SRY gene.

Read the entire article on fragile X and trinucleotide expansion mechanism: Trinucleotide repeat expansion disorders

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The image represents base substitution mutation in DNA. image credit: genome research limited.

Some mutations are stable, it can be inherited from generation to generation. Some mutations are non-stable,  may occur for some time in a single generation.

Stable mutations are generally congenital, and non-stable mutations arise after birth. Stable mutations incorporated into genome it can never be repaired, while unstable mutation can be repaired and passes the wild-type allele back to the population.

Pleiotropy

Mutation in one gene influences more than one trait or phenotype is referred to as pleiotropy. Again recall the situation in sickle cell anaemia. Mutant HBS allele results in anaemic condition as well as protect the heterozygous individual from the malaria parasite. We will discuss pleiotropy broadly in other article.

Different types of Genetic mutations randomly occur in the population. It creates allelic variation in a genome and the new allele originates in the population. Polymorphism is a natural phenomenon. Genetic mutation is occurred to make us adaptive in an adverse environment but it may be harmful sometime. Scientifically, “what we are today, is a result of millions of mutation in the past”.

Read further,

Article written by – Tushar Chauhan 

Categories: All articles, Educational articles, Explore, Mutation Tags: , , , , , , , , ,

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